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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Author

  • Vilhelm Müller
  • Lena K. Nyberg
  • Saair Quaderi
  • Erik Kristiansson
  • Tobias Ambjörnsson
  • Linus Sandegren
  • Fredrik Westerlund

Summary, in English

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.

Department/s

  • Computational Biology and Biological Physics

Publishing year

2016

Language

English

Pages

1561-1562

Publication/Series

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Medical Laboratory and Measurements Technologies

Keywords

  • Antibiotic resistance
  • CRISPR/Cas9
  • Nanofluidics
  • Optical DNA mapping
  • Plasmids

Conference name

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference date

2016-10-09 - 2016-10-13

Conference place

Dublin, Ireland

Status

Published

ISBN/ISSN/Other

  • ISBN: 9780979806490