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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Blinking statistics of a molecular beacon triggered by end-denaturation of DNA

Author

  • Tobias Ambjörnsson
  • Ralf Metzler

Summary, in English

We use a master equation approach based on the Poland–Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore–quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins that selectively bind to single-stranded DNA. We show that such a set-up could be well suited as an easy-to-implement nanodevice for sensing environmental conditions in small volumes.

Publishing year

2005-12-14

Language

English

Pages

4305-4316

Publication/Series

Journal of Physics: Condensed Matter

Volume

17

Issue

49

Document type

Journal article

Publisher

IOP Publishing

Topic

  • Biophysics

Status

Published

ISBN/ISSN/Other

  • ISSN: 0953-8984