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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA mapping

Author

  • Vilhelm Müller
  • Nahid Karami
  • Lena K. Nyberg
  • Christoffer Pichler
  • Paola C. Torche Pedreschi
  • Saair Quaderi
  • Joachim Fritzsche
  • Tobias Ambjörnsson
  • Christina Åhrén
  • Fredrik Westerlund

Summary, in English

Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum β-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.

Department/s

  • Computational Biology and Biological Physics

Publishing year

2016-05-13

Language

English

Pages

322-328

Publication/Series

ACS Infectious Diseases

Volume

2

Issue

5

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Medicinal Chemistry
  • Infectious Medicine

Keywords

  • antibiotic resistance
  • nosocomial outbreak
  • optical DNA mapping
  • plasmids

Status

Published

ISBN/ISSN/Other

  • ISSN: 2373-8227