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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Bacteriophage strain typing by rapid single molecule analysis.

Author

  • Assaf Grunwald
  • Moran Dahan
  • Anna Giesbertz
  • Adam Nilsson
  • Lena K Nyberg
  • Elmar Weinhold
  • Tobias Ambjörnsson
  • Fredrik Westerlund
  • Yuval Ebenstein

Summary, in English

Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base recognition sites of a DNA methyltransferase. Due to the diffraction limit of light, the dense distribution of labels results in a continuous fluorescent signal along the DNA. The amplitude modulations (AM) of the fluorescence intensity along the stretched DNA molecules exhibit a unique molecular fingerprint that can be used for identification. We show that this labelling scheme is highly informative, allowing accurate genotyping. We demonstrate the method by labelling the genomes of λ and T7 bacteriophages, resulting in a consistent, unique AM profile for each genome. These profiles are also successfully used for identification of the phages from a background phage library. Our method may provide a facile route for screening and typing of various organisms and has potential applications in metagenomics studies of various ecosystems.

Department/s

  • Atomic Physics
  • Computational Biology and Biological Physics

Publishing year

2015

Language

English

Pages

117-117

Publication/Series

Nucleic Acids Research

Volume

43

Issue

18

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Biophysics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1362-4962