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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Identifying bacteria using DNA binding maps

Author

  • Gustav Emilsson
  • Adam Nilsson
  • Lena K. Nyberg
  • Charleston Noble
  • Liselott Svensson Stadler
  • Edward R.B. Moore
  • Tobias Ambjörnsson
  • Jonas O. Tegenfeldt
  • Fredrik Westerlund

Summary, in English

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Department/s

  • Synchrotron Radiation Research
  • Computational Biology and Biological Physics
  • Solid State Physics

Publishing year

2013-01-01

Language

English

Pages

473-475

Publication/Series

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Volume

1

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Biophysics

Keywords

  • Bacterial identification
  • Nanofluidics
  • Optical mapping

Conference name

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Conference date

2013-10-27 - 2013-10-31

Conference place

Freiburg, Germany

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781632666246