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Face of Tobias Ambjörnsson. Photo.

Tobias Ambjörnsson

Senior lecturer

Face of Tobias Ambjörnsson. Photo.

Bacterial identification by optical mapping of genomic DNA in nanofluidic channels

Author

  • My Nyblom
  • Vilhelm Müller
  • Anna Johnning
  • Marie Wrande
  • Albertas Dvirnas
  • K. K. Sriram
  • Christian G. Giske
  • Tobias Ambjörnsson
  • Linus Sandegren
  • Erik Kristiansson
  • Fredrik Westerlund

Summary, in English

A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.

Department/s

  • Computational Biology and Biological Physics

Publishing year

2019

Language

English

Pages

821-822

Publication/Series

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Document type

Conference paper

Publisher

Chemical and Biological Microsystems Society

Topic

  • Microbiology in the medical area
  • Infectious Medicine

Keywords

  • Bacterial Identification
  • Nanofluidics
  • Optical DNA Mapping

Conference name

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference date

2019-10-27 - 2019-10-31

Conference place

Basel, Switzerland

Status

Published

ISBN/ISSN/Other

  • ISBN: 9781733419000