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Picture of Patrik Edén

Patrik Edén

Senior Lecturer

Picture of Patrik Edén

Gene expression profiling indicates that immunohistochemical expression of CD40 is a marker of an inflammatory reaction in the tumor stroma of diffuse large B-cell lymphoma

Author

  • Karin Fjordén
  • Patrick Joost
  • Mats Ehinger
  • Patrik Edén
  • Mats Jerkeman
  • Eva Cavallin-Ståhl
  • Johan Linderoth

Summary, in English

Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p = 0.005), which in turn correlates with the amount of infiltrating macrophages and CD4 and CD8 positive T-cells. We postulate that immunohistochemical expression of CD40 mainly reflects the inflammatory status in tumors. A high intratumoral inflammatory reaction may correlate with an increased autologous tumor response, and thereby a better prognosis.

Department/s

  • Breastcancer-genetics
  • Tumor microenvironment
  • Computational Biology and Biological Physics
  • Lymphoma - Clinical Research

Publishing year

2012

Language

English

Pages

1764-1768

Publication/Series

Leukemia & Lymphoma

Volume

53

Issue

9

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Cancer and Oncology

Keywords

  • Diffuse large B-cell lymphoma
  • CD40
  • gene expression profiling
  • tumor
  • stroma

Status

Published

Research group

  • Lymphoma - Clinical Research

ISBN/ISSN/Other

  • ISSN: 1042-8194