Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here:

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Picture of Patrik Edén

Patrik Edén

Senior Lecturer

Picture of Patrik Edén

Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations


  • Anna Andersson
  • Patrik Edén
  • David Lindgren
  • Jens Nilsson
  • Carin Lassen
  • Jesper Heldrup
  • Magnus Fontes
  • Åke Borg
  • Felix Mitelman
  • Bertil Johansson
  • Mattias Höglund
  • Thoas Fioretos

Summary, in English

Hematologic malignancies are characterized by fusion genes of biological/clinical importance. Immortalized cell lines with such aberrations are today widely used to model different aspects of leukemogenesis. Using cDNA microarrays, we determined the gene expression profiles of 40 cell lines as well as of primary leukemias harboring 11q23/MLL rearrangements, t(1;19)[TCF3/PBX1], t(12;21)[ETV6/RUNX1], t(8;21)[RUNX1/CBFA2T1], t(8;14) [IGH@/MYC], t(8;14)[TRA@/MYC], t(9;22)[BCR/ABL1], t(10;11) [PICALM/MLLT10], t(15;17)[PML/RARA], or inv(16)[CBFB/MYH11]. Unsupervised classification revealed that hematopoietic cell lines of diverse origin, but with the same primary genetic changes, segregated together, suggesting that pathogenetically important regulatory networks remain conserved despite numerous passages. Moreover, primary leukemias cosegregated with cell lines carrying identical genetic rearrangements, further supporting that critical regulatory pathways remain intact in hematopoietic cell lines. Transcriptional signatures correlating with clinical subtypes/primary genetic changes were identified and annotated based on their biological/molecular properties and chromosomal localization. Furthermore, the expression profile of tyrosine kinase-encoding genes was investigated, identifying several differentially expressed members, segregating with primary genetic changes, which may be targeted with tyrosine kinase inhibitors. The identified conserved signatures are likely to reflect regulatory networks of importance for the transforming abilities of the primary genetic changes and offer important pathogenetic insights as well as a number of targets for future rational drug design.


  • Division of Clinical Genetics
  • Computational Biology and Biological Physics
  • Breastcancer-genetics
  • Mathematics (Faculty of Engineering)
  • Paediatrics (Lund)
  • The pathogenetic mechanisms behind MLL-rearranged acute leukemia in infancy

Publishing year












Document type

Journal article


Nature Publishing Group


  • Cancer and Oncology


  • cDNA microarray
  • AML
  • CML-BC
  • ALL
  • pediatric leukemia



Research group

  • The pathogenetic mechanisms behind MLL-rearranged acute leukemia in infancy


  • ISSN: 1476-5551