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Anders Irbäck. Photo.

Anders Irbäck

Professor

Anders Irbäck. Photo.

Local Unfolding and Aggregation Mechanisms of SOD1: A Monte Carlo Exploration.

Author

  • Anna Bille
  • Sigurdur Jonsson
  • Mikael Akke
  • Anders Irbäck

Summary, in English

Copper, zinc superoxide dismutase 1 (SOD1) is a ubiquitous homodimeric enzyme, whose misfolding and aggregation play a potentially key role in the neurodegenerative disease amyotrophic lateral sclerosis (ALS). SOD1 aggregation is thought to be preceded by dimer dissociation and metal loss, but the mechanisms by which the metal-free monomer aggregates remain incompletely understood. Here we use implicit solvent all-atom Monte Carlo (MC) methods to investigate the local unfolding dynamics of the β-barrel-forming SOD1 monomer. Although event-to-event variations are large, on average, we find clear differences in dynamics among the eight strands forming the β-barrel. Most dynamic is the eighth strand, β8, which is located in the dimer interface of native SOD1. For the four strands in or near the dimer interface (β1, β2, β7, and β8), we perform aggregation simulations to assess the propensity of these chain segments to self-associate. We find that β1 and β2 readily self-associate to form intermolecular parallel β-sheets, whereas β8 shows a very low aggregation propensity.

Department/s

  • Computational Biology and Biological Physics
  • Biophysical Chemistry
  • MultiPark: Multidisciplinary research focused on Parkinson´s disease

Publishing year

2013

Language

English

Pages

9194-9202

Publication/Series

The Journal of Physical Chemistry Part B

Volume

117

Issue

31

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Other Physics Topics
  • Biophysics
  • Physical Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1520-5207