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Anders Irbäck. Photo.

Anders Irbäck

Professor

Anders Irbäck. Photo.

Two-state folding over a weak free-energy barrier

Author

  • Giorgio Favrin
  • Anders Irbäck
  • Björn Samuelsson
  • Stefan Wallin

Summary, in English

We present a Monte Carlo study of a model protein with 54 amino acids that folds directly to its native three-helix-bundle state without forming any well-defined intermediate state. The free-energy barrier separating the native and unfolded states of this protein is found to be weak, even at the folding temperature. Nevertheless, we find that melting curves to a good approximation can be described in terms of a simple two-state system, and that the relaxation behavior is close to single exponential. The motion along individual reaction coordinates is roughly diffusive on timescales beyond the reconfiguration time for a single helix. A simple estimate based on diffusion in a square-well potential predicts the relaxation time within a factor of two.

Department/s

  • Computational Biology and Biological Physics

Publishing year

2003

Language

English

Pages

1457-1465

Publication/Series

Biophysical Journal

Volume

85

Issue

3

Document type

Journal article

Publisher

Cell Press

Topic

  • Biophysics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1542-0086